Olympus Microscope FV3000

Macro-to-Micro Imaging and Super Resolution Microscopy

The FV3000 microscope’s macro-to-micro workflow provides a roadmap for data acquisition, enabling you to see data in context and easily locate regions of interest for higher resolution imaging.

• Use a low-magnification 1.25X or 2X objective to quickly capture a large field of view (FOV) map of whole specimens
• Identify regions of interest on the Overlay Map, then switch to a higher magnification objective for high-resolution confocal imaging down to 120 nm with Olympus Super Resolution technology (FV-OSR)
• Finalize your acquisition and get publication-ready microscope images with TruSight image processing

Hybrid Scanning for High-Speed Imaging and Increased Productivity

The FV3000 Hybrid Scanner provides two scanners in one for enhanced confocal imaging capabilities.

• The FV3000RS hybrid scan unit uses a galvanometer scanner for precision scanning as well as a resonant scanner, ideal for high-speed imaging of live physiological events
• Capture video-rate images with a large FOV using the resonant scanner, featuring speeds from 30 frames per second (fps) at FN 18 all the way up to 438 fps using clip scanning
• Use the resonant scanner to observe fast phenomena, such as a beating heart, blood flow, or calcium ion (Ca2+) dynamics inside cells
• Switch between the galvanometer scanner and resonant scanner with the click of a button

Accurate Time-Lapse Imaging

Time-lapse experiments require consistent focus and low phototoxicity to the sample.

• Olympus’ TruFocus unit helps maintain focus during live cell imaging despite changes in temperature or added reagents
• The FV3000 microscope’s high-sensitivity detector requires significantly less laser power while the resonant scanner reduces laser illumination time, lowering phototoxicity for more physiologically accurate confocal imaging data

Deep Tissue Observation with Silicone Objectives

The refractive index of silicone oil is close to that of living tissue, enabling high-resolution observation deep inside tissue with minimal spherical aberration.

• Refractive index match delivers an ideal focal volume, resulting in perfect volume reconstruction and enabling high-resolution confocal imaging of large living organisms
• Long working distances enable detailed microscopic imaging at depth
• See data unfold in real-time and easily observe structures with 3D reconstruction software

		FV3000	FV3000RS
Main Laser Combiner	Violet/Visible Light Laser	405 nm: 50 mW, 488 nm: 20 mW, 561 nm: 20 mW, 640 nm: 40 mW One optional laser port for the sub laser combiner or optional laser unit
Optional Laser	Sub Laser Combiner	Maximum 3 laser units as follows: 445 nm: 75 mW, 514 nm: 40 mW, 594 nm: 20 mW, fiber connected to main laser combiner
	Single Laser Unit	445 nm: 75 mW, 514 nm: 40 mW, or 594 nm: 20 mW, directly connected to main laser combiner
Laser Light Control		Main laser combiner with implemented AOTF system, ultra-fast intensity modulation with individual laser lines, additional shutter control continuously variable (0.1%–100%, 0.1% increments) 10% or 100% maximum laser power using an ND filter
Scanner	Scanning Method	2 silver-coated galvanometer scanning mirrors	2 silver-coated galvanometer scanning mirrors 1 silver-coated resonant and 1 silver-coated galvanometer scanning mirrors
	Galvanometer Scanner (Normal Imaging)	Scanning Resolution: 64 × 64 to 4096 × 4096 pixels Scanning Speed (One Way): 512 × 512 with 1.1 s–264 s. pixel time: 2 µs–1000 µs Scanning Speed (Round Trip): 512 × 512 with 63 ms–250 ms, 256 × 256 with 16 ms–125 ms Optical Zoom: 1X–50X in 0.01X increments Scan Rotation: Free rotation (360 degree) in steps of 0.1 degree Scanning Mode: PT, XT, XZ, XY, XZT, XYT, XYZ, XYλ, XYZT, XYλT, XYλZ, XYλZT ROI scanning, rectangle clip, ellipse, polygon, free area, line, free line and point, tornado mode only for stimulation
	Resonant Scanner (High-Speed Imaging)	–	Scanning Resolution: 512 × 32 to 512 × 512 pixels Scanning Speed: 30 fps at 512 × 512, 438 fps at 512 x 32 Optical Zoom: 1X–8X in 0.01X increments Scanning Mode: XT, XZ, XY, XZT, XYT, XYZ, XYλ, XYZT, XYλT, XYZ, XYλZT ROI scanning, rectangle clip, line
	Pinhole	Single motorized pinhole, pinhole diameter ø50–800 µm (1 µm steps)
	Field Number (FN)	18
	Dichromatic Mirror Turret	8 positions (high-performance DMs and 10/90 mirror)
	Optional Unit for Scanner	Laser power monitor, optional laser port
High-Sensitivity Spectral Detector	Detector Module	Cooled GaAsP photomultiplier, 2 channels
	Spectral Method	Motorized volume phase holographic transmission diffraction grating, motorized adjustable slit, selectable wavelength bandwidth: 1–100 nm, wavelength resolution: 2 nm
	Dichromatic Mirror Turret	8 positions (high-performance DMs and mirror)
System Control	Control Unit	OS: Windows® 7 Professional 64-bit (English version), Windows 10 Professional 64-bit ; built-in dedicated I/F board and hardware sequencer for precise imaging timing
	Display	30- or 32-inch monitor (WQUXGA 2560 × 1600)
Fluorescence Illumination Unit		External fluorescence light source, fiber adaptor to the optical port of the scan unit, motorized switching between the LSM light path and fluorescence illumination
Transmitted Light Detector Unit		Module with integrated external transmitted light photomultiplier detector and LED lamp, motorized switching

Microscope

	Inverted frame	Upright frame (for imaging)	Upright frame (for electrophysiology)
Microscope Frame	Motorized inverted microscope  IX83 (IX83P2ZF)	Motorized fixed stage upright microscope  BX63L	Motorized fixed stage upright microscope  BX63L
Revolving Nosepiece	Motorized sextuple revolving nosepiece	Motorized septuple revolving nosepiece	Coded swing nosepiece  Coded slider nosepiece
Condenser	Motorized long working distance condenser	Motorized universal condenser	Manual long working distance condenser
Focus Stroke	Built-in motorized nosepiece focus  Stroke: minimum increment: 0.01 μm

Software

Basic Features	GUI designed for darkroom environment. User-arrangeable layout. Acquisition parameter reload features. Hard disk recording capability; adjust laser power and HV with Z-stack acquisition.    Z-stack with alpha blending, maximum-intensity projection, iso-surface rendering.
2D Image Display	Each image display: single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ), LUT: individual color setting, pseudo-color, comment: graphic and text input.
3D Visualization and Observation	Interactive volume rendering: volume rendering display, projection display, animation displayed. 3D animation (maximum intensity projection method, α blending) 3D and 2D sequential operation function.
Image Format	OIR image format  8/16-bit gray scale/index color, 24/ 32/ 48-bit color, JPEG/ BMP/ TIFF image functions, Olympus multi-tif format.
Spectral Unmixing	Fluorescence spectral unmixing modes (up to 16 channels)
Image Analysis	Region and line measurements, intensity plot over time/Z, colocalization analysis.
Statistical Processing	2D data histogram display.
Optional Software	Motorized-stage control   Mapping and multipoint simulation  Sequence manager  Virtual channel acquisition  Microplate navigation  Remote development kit  Super resolution imaging (FV-OSR)  Digital camera control function  Deconvolution  FRET and FRAP analysis  Automatic object measurement and classification  Object tracking