Orbital shaken bioreactor for single-use bags
The SB200-X is an orbital shaken bioreactor for the cultivation of human, mammalian and plant cells in a single-use bag. A working volume from 50 litres up to 200 litres for the SB200-X ensures easy scale-up from microtiter plates to shaking asks and all the way up to 200 litres. This user friendly system can be easily deployed in research, process development or production.
The SB200-X was developed and tested in collaboration with ExcellGene SA (Monthey, Switzerland) and the Swiss Federal Institutes of Technology EPFL (Lausanne, Switzerland).
Performance characteristics
- Large capacity (200L working volume) single use bioreactor system
- Cultivation of human, mammalian and plant cells
- Fast, simple set up
- Easy scale-up
- Online measurement of pH and DO
Orbital shaken bioreactor for single-use bags
The SB200-X is an orbital shaken bioreactor for the cultivation of human, mammalian and plant cells in a single-use bag. A working volume from 50 litres up to 200 litres for the SB200-X ensures easy scale-up from microtiter plates to shaking asks and all the way up to 200 litres. This user friendly system can be easily deployed in research, process development or production.
The SB200-X was developed and tested in collaboration with ExcellGene SA (Monthey, Switzerland) and the Swiss Federal Institutes of Technology EPFL (Lausanne, Switzerland).
Performance characteristics
- Large capacity (200L working volume) single use bioreactor system
- Cultivation of human, mammalian and plant cells
- Fast, simple set up
- Easy scale-up
- Online measurement of pH and DO
Bag
Single-use bag for a fast and simple set up
The vessel accepts a single-use bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO.
Orbital motion
Orbital motion
The orbital motion of the SB200-X ensures efficient liquid mixing. It allows high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB200-X uses the unique Kuhner direct drive which provides acurate control of the orbital speed.
Scale-Up
Trouble free scale-up
Another advantage of the SB200-X is easy scale-up from microtiter plates all the way up to 200 litres. The consistent hydro-dynamics of shaken bioreactors provide reproducible cultivation conditions throughout the volume range.
Control unit
Control unit and online measurement
The bespoke control unit, designed by Kuhner, uses the latest touch-screen technology and Kuhner Insight software for data recording, calibration, programming and controlling. Technical controls for 21 CFR Part 11 compliance are built into the software.
The SB200-X can be heated or cooled. Temperature ramps are easily programmed and processed.
Duplex online measurement of each parameter (dissolved oxygen and pH value) is available ensuring greater process safety.
Pumping system
The control unit incorporates two small and one big peristaltic pump. This enables simple regulation of the pH value, feeding and the transfer of the culture media (scale-up or processing).
Gas mixing
The SB200-X has an integrated gas mixing device (FlowCon).
Whether stabilizing the pH with CO2, or preventing low dissolved oxygen concentrations, the gas mixer meets all user needs. Four mass flow controllers ensure highly accurate and reproducible gas mixing. An integrated pressure sensor prevents over pressure in the bioreactor by reducing the gas flow in the first step (1. limit) and shutting off the gas flow in the second step (2. limit). Step limits can be freely programmed by the user. Integrated air filters and back pressure valves ensure interference free and long operating times for the FlowCon
Filter heater
A height adjustable filter heater, with up to two exhaust filters, is installed above the vessel. Special emphasis has been placed on developing a user friendly unit. The filter heater combats any water vapour condensation in the outlet air which might cause the membranes to block. The temperature is monitored and controlled by Kuhner Insight software.
Shaker
|
|
SMX7100 |
|
Shaker speed |
max. 80 rpm |
|
Shaker diameter |
50 mm (orbital motion) |
|
Weight |
approx. 400 kg without liquid |
|
Accuracy, absolute |
0.1 rpm |
|
Setting, digital |
1 rpm |
|
Active brake |
adjustable |
|
Interface |
CAN-Bus, RS232 |
|
Temperature |
up to 40°C |
|
Cooling |
cooling coils are incorporated for connection to an external cooling system (pressure < 0.2bar) |
Dimensions (in mm) shaker
Mains connection
|
SMX1021 |
220 – 240 V / 50-60 Hz |
|
SMX1023 |
110 – 120 V / 50-60 Hz |
|
SMX1024 |
95 – 105 V / 50-60 Hz |
Reader Box-X
|
pH measurement |
principle: optical chemosensor |
|
Range |
pH 5.5 – pH 8.5 |
|
Accuracy (chemosensor) |
± pH 0.05 at pH 7 with one point calibration ± pH 0.10 at pH 7 with pre-calibration |
|
Drift |
< pH 0.005 per day |
|
Temperature range |
up to 50°C |
|
|
|
|
DO measurement |
principle: optical chemosensor |
|
Range |
0% – 100% DO |
|
Accuracy (chemosensor) |
± 0.1% O2 at 20.9% O2 |
|
Accuracy (system) |
± 10% DO |
|
Drift |
< 0.015% O2 per day |
|
Temperature range |
up to 50°C |
Filter heater
SMX7120 | |
Capacity | 2 exhaust filters |
Temperature range | Ambient temperature up to 45°C, |
Control unit
Dimensions (in mm)
Kuhner Insight Software
|
Software |
for monitoring, calibration, controlling |
|
Monitoring |
O2, pH, shaking speed, temperature |
|
Calibration |
pre-calibrated
sensors in bag |
|
Recalibration |
one point recalibration is possible |
|
Applicable |
XP/Vista/Win7&8 |
|
GMP |
21 CFR Part 11 compliance
(audit trail) |
Pumps
|
2 small pumps (A+B) |
Watson-Marlow 313VBM/D |
|
Gear |
10 |
|
Maximum Speed |
100 rpm |
|
Power |
70 W |
|
Rollers |
3 |
|
Tube |
1.6 mm wall tubing |
|
Delivery Pressure |
max. 2bar |
|
|
|
| 1.6 mm wall tubing Bore diameter (mm) |
Flow rates (ml/min at 100 rpm) |
|
0.5 |
3.4 |
|
0.8 |
7 |
|
1.6 |
27 |
|
3.2 |
100 |
|
4.8 |
220 |
|
6.4 |
360 |
|
8.0 |
500 |
Gas mixing
|
Number of mass flow controllers |
up to 4 |
|
Ambient conditions |
5-40 [°C], max. 85 [%rH] |
|
Input |
up to 4 gases, push in connector 6 [mm] (air, oxygen,nitrogen, carbon dioxide) (gas mixtures can also be connected) |
|
Input pressure |
1-2 bar |
|
Output |
1, (tube fitting for tube ID 13 [mm]) |
|
Flow rate |
0 – 20 [sL/min] |
|
Accuracy |
±1.5% from the end value |
|
Pressure sensor |
integrated, 0-400 [mbar], programmable prevents overpressure in the connected vessel |
|
Air filter |
integrated, prevents blocking the mass flow controllers |
|
Back pressure valve |
integrated, prevents reflux into the mass flow controllers |
|
Controlled filter heating box |
for the outlet filters, prevents blocking of the filters caused by condensation (only air inlet and outlet with silicon tubing) |
|
C-Flex material |
for the tubing, enables the user to connect additional components via tube welding |